Figure 7.

Inhibition of dynein-mediated nuclear targeting blocks the incoming viruses in the cytoplasm but does not prevent the capsid rearrangement, phosphorylation and uncoating (A) Effect of the dynein inhibitor ciliobrevin D (CbD; 100 µM) on conformational epitope integrity (Caps/PLA2 ratio). (B) Effect of CbD on phosphorylation and DNA externalization. (C) Effect of CbD on the nuclear accumulation of B19V DNA. After 5 min (background control) and 3 h pi, cells were washed and the amount of viral DNA was quantified by qPCR. Additionally, cells were incubated for two additional hours in the presence or absence of CbD. All values of qPCR correspond to geq/µL. (D) Effect of CbD on B19V infection. Cells were infected in the presence of CbD for the indicated times and the amount of NS1 mRNA was quantified by RT-qPCR at 22 h pi. Values represent NS1 mRNA copies/µL.