Table 3.
Primers used in this study.
| Primer | Sequence (5′-3′) | Use |
|---|---|---|
| FGSG_TRI5-qF | CACTTGTCAAGGAGCACTTTC | qRT-PCR for determining the expression of TRI5 (FGSG_03537) |
| FGSG_TRI5-qR | TGCTCAATCCAACATCCCTC | |
| Actin-qF | ATCCACGTCACCACTTTCAA | qRT-PCR for inter-reference actin |
| Actin-qR | TGCTTGGAGATCCACATTTG | |
| FGSG_00500-qF | CGACCTCCACGACAACA | qRT-PCR for determining the expression of hexokinase (FGSG_00500) |
| FGSG_00500-qR | GATAGCAGCAACGCCACA | |
| FGSG_09456-qF | GATAGATTGGAGAGCCGAGAGA | qRT-PCR for determining the expression of 6-phosphate fructokinase (FGSG_09456) |
| FGSG_09456-qR | GAGGTGCTGGATACACTTGATG | |
| FGSG_07528-qF | GAGATCCGAACTGGTAAGACTC | qRT-PCR for determining the expression of pyruvate kinase (FGSG_07528) |
| FGSG_07528-qR | CGTCAGAAGCGGTAGCATAA | |
| TRI1-GFP-F | ACTCACTATAGGGCGAATTGGGTACTCAAATTGGTTTTGTGAGTAGGCCTCATA | A pair of PCR primers to amplify TRI1(FGSG_00071) fragments used for construction of the TRI1-GFP, vector under its own promoter |
| TRI1-GFP-R | CACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACGTCATCCTGTACCAATTCCAATCG |