Table 1.

Particle distribution of HSP variants determined by negative stain EM

HSP variant	24 subunit	36 subunit	48 subunit
P1	-	7.5	92.5
Var-1	-	15.1	84.7
Var-2	3.1	57.9	39.0
Var-3	8.5	91.5	-
Var-4	-	100	-
Var-5	12.0	86.2	3.8
Var-6	1.0	97.4	1.6
Var-7	53.8	41.7	6.6
Var-8	32.9	53.1	7.4

The Hsp16.5 mutant datasets were refined against three representative structures of 24, 36, and 48 subunits with the EMAN multi-refine subroutine in order to partition individual particle images into one of three classes. For each Hsp16.5 mutant the percentage of particles placed into each of the three classes is shown.