Fig. 3.

Triacylglycerol (TAG) lipid species have significantly different profiles in peri-gonadal adipose tissue from reproductively young and old mice. Volcano plots showing the log fold-change versus statistical significance (log P value) for the (a) Triacylglycerol (TAG) 1 and (b) Triacylglycerol (TAG) 2 methods. A 1.5-fold change and p ≤ 0.05 were used as the threshold for significance. 2D Principal component analysis (PCA) plots show the separation of triacylglycerol lipids in peri-gonadal adipose tissue samples based on age for the (c) TAG 1 and (d) TAG 2 methods. The data for reproductively young mice are shown in green and old mice are shown in red. Heatmaps representing color-coded profiling of the 25 most abundant triacylglycerol lipid species in peri-gonadal adipose tissue from reproductively young (green) and old (red) mice in the (e) TAG 1 method and (f) TAG 2 method. The top 25 lipid species corresponding to the numbers in the heatmaps are listed in Table 1