Figure 4.
Effects of VP and antagonist CVP on infected alveolar macrophages. (A) Number of cultivable intracellular bacilli expressed as CFU per milliliter at 1 and 24 h post-infection in RPMI controls (black bars), incubated with three different doses of DdAVP (1 × 10−8 M, white bars; 1 × 10−7 M gray bars, and 1 × 10−6 M dark gray bars) or CVP (1 × 10−6 M, lined bars). (B) Effect of VP or CVP on mycobacterial killing by MHS cells at 24 and 72 h post-infection. (C) Quantification of IL-6 and TNFα (D) in pools of supernatants of infected MHS cells detected by ELISA. Data are expressed as means ± SEM of three different wells at each point. Asterisk represent statistical significance (*P < 0.05, two-way ANOVA).