Figure 3.

Discrete gene expression regulates MG cell compartment morphology. (a) Heatmap to show the relative gene expression for genes tested. These were all screen in F0 CRISPR injected mutants. (b) Summary of phenotypes observed for genes enriched across windows of MG differentiation. Red—phenotype, blue—no‐phenotype. (c) lamb4 mutants have defects in the apico‐basal distribution of MG cell bodies only. (d) dcaf8 mutants have defects in the OLM and OPL. (e) mmp28 CRISPR injected mutants have defects in the ILM only. (f) egr1 mutants have defects in the IPL and OPL. (g) slitkr2 mutants have defects in the OPL layer only. (h) icn2 mutants have tiling defects only. 1. Frequency (%) of phenotypes observed in each MG compartment in F0 CRISPR screen 2. Scale bars = 8 μm