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. 2019 Feb 28;49(5):801–811. doi: 10.1002/eji.201847888

Figure 1.

Figure 1

Expression of MHC II on M2‐macrophages and TC‐Mϕ treated with IMQ or Poly(I:C). Macrophages were in vitro differentiated by stimulating monocytes with 25 ng/mL of rhM‐CSF for 6 days, and then polarized with 100 ng/mL of LPS and 50 ng/mL of IFNγ (M1), 20 ng/mL of IL‐4 (M2) or medium (M0) for 24 h. TC‐Mϕ were prepared by stimulating monocytes for 6 days with 30% of tumor conditioned‐medium (from the tumor cell lines PANC1 or PT45). (A) Expression of MHC II analyzed by flow cytometry in differently polarized macrophages; the results are expressed as fold increase (Mean fluorescent intensity), relative to M0 macrophages; mean ± SEM are indicated from two to four independent experiments (total 3–7 donors); each symbol corresponds to a different blood donor). (B) Representative flow cytometry histograms of MHC II expression in M2 macrophages (left panel) and in TC‐Mϕ (right panel) treated with IMQ (10 μg/mL) or Poly(I:C) (20 μg/mL) for 72 h. (C–E) MHC II expression in M2 macrophages (C) and in TC‐Mϕ (D–E) treated with IMQ or Poly(I:C) for 72 h. Results are expressed as fold increase relative to unstimulated cells. *p < 0.03, **p < 0.002, and ***p < 0.0002 versus nontreated macrophages.