Table 2.
mRNAscope probes
| Probe | Protein/peptide | Channel numbers | Catalogue numbers | Z‐pair number | Target region |
|---|---|---|---|---|---|
| Cck | CCK | 1, 2 | 402271 | 12 | 23–679 |
| Slc17a6 | VGLUT2 | 2 | 319171 | 20 | 1,986–2,998 |
| Prkcg | PKCγ | 1 | 417911 | 20 | 685–2,438 |
| Trh | TRH | 2, 3 | 436811 | 20 | 128–1,280 |
| Nts | Neurotensin | 3 | 420441 | 20 | 2–1,188 |
| Tac1 | Substance P | 1 | 410351 | 15 | 20–1,034 |
| Tac2 | NKB | 3 | 446391 | 15 | 15–684 |
| RNAscope multiplex positive control (Polr2a, Ppib, Ubc) | Polr2a: DNA‐directed RNA polymerase II subunit RPB1; Ppib: Peptidyl‐prolyl cis‐trans isomerase B; Ubc: Polyubiquitin‐C | 1,2,3 | 320881 | 20 15 n/a |
2,802–3,678 98–856 34–860 |
| RNAscope multiplex negative control (dapB) | dapB: 4‐hydroxy‐tetrahydrodipicolinate reductase (derived from Bacillus subtilis) | 1,2,3 | 320871 | 10 | 414–862 |