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Effects of NF-kB ablation on basal expression of the indicated “oxidant” genes. mRNA levels of Nox2 and Nox4 isoforms and p47 Nox complex component, quantified by qPCR in wt or NFkB DN U937 cells. Data were normalized to 18S rRNA and results are expressed as relative fold change versus Nox2 mRNA levels in wt control cells. Mean values + S.D. from three independent experiments performed in duplicates are depicted. * p < 0.001. na: no amplified cDNA detected.
