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. 2019 May 10;11(5):428. doi: 10.3390/v11050428

Figure 4.

Figure 4

ROS production in THP-1 cells exposed to HSV-1. Cells were preloaded with DCFH-DA 10 µM, infected with HSV-1 and monitored for ROS production as described in Figure 1. (A) Brightfield (total cells) and FITC channel (ROS positive cells) from THP-1 cells infected or mock-infected with HSV-1, MOI 50, for 0.5 h. Digital images were captured by a 40× objective (Zeiss Axio Observer Z1 microscope). Scale bar = 10 μm. (B) % of DCF fluorescence positive cells (mean + S.D) obtained by fluorescence microscope analysis. Frames randomly captured by 20× objective from two separate experiments (2 frames each, total n = 4) were evaluated. * p < 0.001. vs control (h 0).