Figure 6.

Effects of selenium-based antioxidants on early ROS induction by HSV-1. U937 cells were incubated with SeDA4 (20 µM), SeMC (20 µM) or vehicle alone for 48 h and then preloaded with DCFH-DA before exposure to HSV-1 (MOI = 50 PFU/cell) for a further 30 min and successive fluorescence microscopy analysis. (A) Representative images in bright-field (upper panels) and FITC (bottom panels) of cells subjected to different experimental conditions are displayed. Objective 20×, scale bar = 50 μm. (B) % of DCF fluorescence positive cells (mean + S.D) from 6 frames randomly selected from two independent experiments (3 frames each). * p < 0.001 vs. control HSV-1-infected cells (vehicle).