Figure 8.

Effects of selenium-based antioxidants on monocytic cells with abrogated NF-KB activity and exposed to HSV-1. U937-DN-IkB were incubated with SeDA4 (20 µM), SeMC (20 µM) or vehicle alone for 48 h and then preloaded with DCFH-DA before infection with HSV-1 (MOI = 50 PFU/cell) and successive fluorescence microscopy analysis. (A) Representative images of cells collected at 2 h post infection. 20× objective. Scale bar = 50 μm. (B) Quantification of results shown in A by analyses from 8 frames randomly selected from two separate experiments (4 frames each). (C) Evaluation of gD-HSV-1 expressing cells by immunofluorescence microscopy analysis of cells collected at 20 h post infection. Each bar represents the mean value + S.D. of three independent experiments. No significant differences among infected cells either for ROS production (B) or for infectivity rate (C).