Figure 9.

Effects of selenium-based antioxidants on HSV-1-induced NF-kB activation. Nuclear extracts from U937 cells treated with SeDA4 (20 µM), SeMC (20 µM) or vehicle alone for 48 h and then exposed or mock-exposed to HSV-1 MOI 50 for further 1 h, were prepared for NF-kB DNA activity by EMSA. (A) EMSA gel from one representative experiment. The position of NF-kB DNA shift is indicated. (B) Quantitative evaluation of EMSA by densitometry image analysis. Results are expressed as fold change in shift band intensities from all sample versus corresponding mock-infected, vehicle treated samples. Each bar represents the mean value + S.D. of three independent experiments. * P < 0.001 vs mock-infected, vehicle treated samples.