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. 2019 Jun 6;13:230. doi: 10.3389/fncel.2019.00230

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Copyright © 2019 Stone, England and O’Sullivan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of dexamethasone on transepithelial resistance (TEER) as a measure of barrier tightness in (A) a two cell (astrocytes and HBMECs), (B) three cell (astrocytes, HBMECs, and pericytes), and (C) four cell model (astrocytes, HBMECs, pericytes, and neurons). Dexamethasone (1 μM) was added to the luminal side and used as positive control that is known to decrease permeability, thus increase TEER. Data represented as mean ± SEM, n = 4 from two experimental repeats. Statistical analysis was conducted using 2-way ANOVA with Sidak’s multiple comparisons test, ^∗P < 0.05 was considered significant.