Table 1.
Primers used for RT-qPCR studies.
| Symbol | Gene bank |
Primer sequence (5′->3′) Fw/Rv |
Amplicon length (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| Nr3c2 | NM_013131.1 | CCAAAGGCTACCACAGTCTC/ TCCCAGACCGACTATTGTCT |
240 | 60 |
| Nr3c1 | NM_012576.2 | ACAGCTCACCCCTACCTTGGT/ CTTGACGCCCACCTAACATGT |
134 | 60 |
| Col3a1 | NM_032085.1 | CTTCTCACCCTGCTTCACCC/ GGGCAGTCTAGTGGCTCATC |
194 | 60 |
| Kall | NM_031523.1 | GCATCACACCTGACGGATTG/ GGCCTCCTGAGTCACCCTTG |
171 | 60 |
| Opn | NM_012881.2 | AGTGGTTTGCTTTTGCCTGTT/ TCAGCCAAGTGGCTACAGCAT |
122 | 60 |
| At1r | NM_031009.2 | CAAAAGGAGATGGGAGGTCA/ TGACAAGCAGTTTGGCTTTG |
254 | 60 |
| Tgfb | NM_021578.2 | GCCAGATCCTGTCCAAACTAA/ TTGTTGCGGTCCACCATTA |
202 | 60 |
| Eca | NM_012544.1 | AACACGGCTCGTGCAGAAG/ CCTGCTGTGGTTCCAGGTACA |
79 | 60 |
| Cox-2 | NM_017232.3 | TGTATGCTACCATCTGGCTTCGG/ GTTTGGAACAGTCGCTCGTCATC |
94 | 60 |
| Atp1a1 | NM_012504.1 | CCCAAAACGGACAAACT/ GCACTACCACGATACTGAC |
274 | 60 |
| Romk | NM_017023.2 | CACTGTGCCATGTGCCTCTA/ ATCTGGGTGTCGTCCGTTTC |
113 | 60 |
| Ncc | NM_019345.3 | GAGAACGGCACACCCATTG/ GACAAGAAAGAACAGCACCTGG |
135 | 60 |
| Nkcc2 | NM_001270618.1 | GCAGGGATCTTTTCGGCAAC/ CTCTCAGGGGCTCGTTGTTT |
148 | 60 |
| Aqp2 | NM_012909.2 | TCACTGGGTCTTCTGGATCG/ CGTTCCTCCCAGTCGGTGT |
147 | 60 |
| Hsd11b2 | NM_017081.2 | CGCGAATGTATGGAGGTG/ CAGTTGCTTGCGCTTCTC |
291 | 60 |
| Nhe3 | NM_012654.1 | ATGGAGAATCTGGCACAC/ TGGCACCCTGGATAGGAT |
213 | 60 |
| Renin | NM_012642.4 | GCTACATGGAGAATGGGACTGAA/ ACCACATCTTGGCTGAGGAAAC |
79 | 60 |
| Per2 | NM 031678 | CACCCTGAAAAGAAAGTGCGA/ CAACGCCAAGGAGCTCAAGT |
148 | 62 |
| Bmal1 | NM024362.2 | CCGATGACGAACTGAAACACCT/ TGCAGTGTCCGAGGAAGATAGC |
215 | 64 |
| Hprt | NM_012583.2 | CCATCACATTGTGGCCCTCT/ TATGTCCCCCGTTGACTGGT |
166 | 60 |
| Gapdh | NM_017008.4 | CTCCCTCAAGATTGTCAGCA/ CCACAGTCTTCTGAGTGGCA |
140 | 60 |