Table 2.
Statistical parameters for clock-time related changes in kidney mRNA expression (2- ΔΔCt) of LD and CPS adult rats at 90 days of age.
| LD group | CPS group | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| ANOVA |
Cosine wave fitting |
Relative expression |
ANOVA |
Cosine wave fitting |
Relative expression |
|||||
| F | Tukey's | R2 | ϕ | 24 h mean | F | Tukey's | R2 | ϕ | 24 h mean | |
| CLOCK GENES | ||||||||||
| Bmal1 | F(5.18) = 63.6 | 08 ≠ 20 h | 0.91* | 22.17 h | 0.88 ± 0.69 | F(5.16) = 11.2 | 08 ≠ 20 h† | 0.69* | 22.23 h | 0.95 ± 0.72 |
| Per2 | F(5.20) = 7.9 | 08 ≠ 24 h† | 0.47* | 14.46 h | 0.65 ± 0.28 | F(5.16) = 16.6 | 08 ≠ 20 h† | 0.71* | 13.56 h | 0.85 ± 0.54 |
| TRANSPORTERS AND CHANNELS | ||||||||||
| Sgk1 | F(6.25) = 10.1 | 12 ≠ 04 h† | 0.60* | 01.38 h | 0.99 ± 0.45 | F(6.24) = 4.9 | 12 ≠ 04 h† | 0.51* | 00.45 h | 0.97 ± 0.52 |
| αEnac | F(6.21) = 3.3 | n.s. | 0.41* | 02.16 h | 0.86 ± 0.21 | F(6.21) = 11.4 | 08 ≠ 20 h† | 0.60* | 23.09 h | 1.00 ± 0.49 |
| Nhe3 | F(6.17) = 3.6 | 08 ≠ 24 h† | 0.44* | 21.23 h | 0.80 ± 0.19 | F(5.20) = 1.5 | n.s. | 0.54* | 23.35 h | 0.97 ± 0.28 |
| Atp1a1 | F(5.18) = 7.6 | 08 ≠ 20 h† | 0.31* | 20.34 h | 2.47 ± 0.62 | F(5.17) = 2.7 | n.s. | 0.24 | n.s. | 2.03 ± 0.75 |
| Ncc | F(5.16) = 12.9 | 12 ≠ 22 h† | 0.04 | n.s. | 0.83 ± 0.4 | F(5.15) = 2.8 | 08 ≠ 12 h† | 0.31 | n.s. | 1.38 ± 0.38 |
| Nkcc2 | F(5.19) = 2.5 | 08 ≠ 16 h† | 0.28 | n.s. | 1.04 ± 0.45 | F(5.15) = 2.7 | n.s. | 0.02 | n.s. | 1.09 ± 0.36 |
| Romk | F(6.20) = 1.6 | n.s. | 0.21 | n.s. | 1.35 ± 0.68 | F(6.20) = 2.9 | n.s. | 0.21 | n.s. | 1.17 ± 0.47 |
| Aqp2 | F(6.19) = 1.2 | n.s. | 0.15 | n.s. | 1.09 ± 0.50 | F(6.19) = 0.8 | n.s. | 0.01 | n.s. | 1.12 ± 0.29 |
| ENZYMES | ||||||||||
| Hsd11b2 | F(5.18) = 2.3 | 12 ≠ 20 h† | 0.24 | n.s. | 1.21 ± 0.54 | F(5.16) = 6.2 | 08 ≠ 20 h† | 0.16 | n.s. | 1.69 ± 0.77 |
| Renin | F(5.22) = 3.1 | 08 ≠ 20 h† | 0.25 | n.s. | 0.74 ± 0.17 | F(5.17) = 0.9 | n.s. | 0.12 | n.s. | 1.03 ± 0.19‡ |
| Kall | F(6.20) = 3.7 | n.s. | 0.08 | n.s. | 0.65 ± 0.30 | F(6.20) = 11.9 | 08 ≠ 24 h† | 0.27 | n.s. | 0.78± 0.59 |
| Cox-2 | F(6.22) = 7.2 | 08 ≠ 20 h† | 0.26 | n.s. | 1.13 ± 0.71 | F(6.20) = 3.9 | n.s. | 0.23 | n.s. | 1.35 ± 0.74 |
| Eca | F(6.22) = 3.5 | 08 ≠ 16 h† | 0.33 | n.s. | 1.33 ± 0.61 | F(6.20) = 4.5 | 08 ≠ 24 h† | 0.13 | n.s. | 1.6 ± 0.86 |
| RECEPTORS | ||||||||||
| At1r | F(5.20) = 9.2 | 08 ≠ 20 h† | 0.37* | 12.49 h | 0.74 ± 0.40 | F(5.16) = 2.4 | 08 ≠ 12 h† | 0.19 | n.s. | 0.71 ± 0.33 |
| Nr3c1 | F(5.20) = 4.7 | 08 ≠ 20 h† | 0.43* | 13.09 h | 0.92 ± 0.16 | F(5.18) = 1.0 | n.s. | 0.13 | n.s. | 0.96 ± 0.10 |
| Avpr2 | F(6.21) = 1.8 | n.s. | 0.34* | 04.04 h | 1.35 ± 0.45 | F(6.22) = 0.1 | n.s. | 0.00 | n.s. | 1.40 ± 0.16 |
| Nr3c2 | F(5.20) = 3.6 | n.s. | 0.16 | n.s. | 0.84 ± 0.09 | F(5.16) = 0.8 | n.s. | 0.09 | n.s. | 0.95 ± 0.08 |
n.s., not significant;
*
, Significant fitting to cosine curve; P < 0.05 Pearson's r.
†
, significant differences from other clock times; P < 0.05 Tukey's.
‡
, different from LD, P < 0.05. un paired t-test.
ϕ, clock time at acrophases.