Table 4.
C. fetus-specific allelic isoform list
| Locus | Full name/product (ORF Locus tag in LMG 6442) |
Calc. average mass [Da] |
Frequency in database | ||
|---|---|---|---|---|---|
| RpmJ/L36 | |||||
| Sequence | MKVRPSVKKMCDKCKIVKRKGIVHVICENPKHKQRQG (37aa) | ||||
| 1a | reference isoform LMG 6442 (NCTC 10842) | 4331.35 | ±0.00 | 100.000% (67/67) | |
| RpmH/L34 | |||||
| Sequence | MKRTYQPHKTPKKRTHGFRGRMKTKNGRKVINARRAKGRKRLAA (44aa) | ||||
| 1a | reference isoform LMG 6442 (NCTC 10842) | 5217.26 | ±0.00 | 47.761% (32/67) | |
| 2a | MKRTYQPHKTPKKRTHGFRERMKTKNGRKVINARRAKGRKRLAA(44aa) | 5289.32 | +72.06 | 32.836% (22/67) | |
| 3 | MKRTYQPHKTPKKRTHGFRERMRTKNGRKVLNARRAKGRKRLAA(44aa) | 5317.33 | +100.07 | 19.403% (13/67) | |
| RpmF/L32-M] | |||||
| Sequence | (M)AVPKRRVSHTRAAKRRTHYKVTLPMPVKDKDGSWKMPHRINKTTGEYa (48aa) | ||||
| 1a | reference isoform LMG 6442 (NCTC 10842) | 5530.47 | ±0.00 | 77.941% (53/68) | |
| 2 | (M)AVPKRRVSHTRAAKRRTHYKVTLPMPVKDKDGSWKMPHRMNKTTGEY (48aa) | 5548.50 | +18.03 | 17.647% (12/68) | |
| 3 | (M)AVPKRRVSHTRAAKRRTHYKVTLPMPVKDKDGSWKMPHRINKITGEY (48aa) | 5542.52 | +12.05 | 1.471% (1/68) | |
| 4 | (M)AVPKRRVSHTRAAKCRTHYKVTLPMPVKDKDGSWKMPHRINKTTGEY (48aa) | 5477.42 | –53.05 | 1.471% (1/68) | |
| 5 | (M)AVPKRLVSHTRAAKRRTHYKVTLPMPVKNKDGSWKMPHRINKTTGEY (48aa) | 5486.45 | –44.02 | 1.471% (1/68) | |
| 6a | (M)AVPKRRVSHTRAAKRRTHYKITLPMPVKDKDGSWKMPHRINKTTGEY (48aa) | 5544.49 | +14.02 | New sequence | |
| RpmG/L33-M | |||||
| Sequence | (M)ASANRVKIGLKCAECNDINYTTTKNSKTTTEKLELKYCPRLKKHTVHKEVKLK (55aa) | ||||
| 1a | reference isoform LMG 6442 (NCTC 10842) | 6205.31 | ±0.00 | 46.970% (31/66) | |
| 2 | (M)ASANRIKIGLKCVECGDINYTTTKNSKKTTEKLELKKYCPRLKKHTEHKEVKLK (55aa) | 6247.39 | +42.08 | 18.182% (12/66) | |
| 3a | (M)ASANRVKIGLKCAECNDINYTTTKNSKKTTEKLELKKYCPRLKKHTVHKEVKLK (55aa) | 6232.38 | +27.07 | 33.333% (22/66) | |
| 4 | (M)ASVNRIKIGLKCVECGDINYTTTKNSKKTTEKLELKKYCPRLKKHTEHKEVKLK (55aa) | 6275.44 | +70.13 | 1.515% (1/66) | |
| 5a | (M)ASANRVKIGLKCAECNDINYTTTKNSKTTTEKSELKKYCPRLKKHTVHKEVKLK (55aa) | 6179.23 | –26.08 | New sequence | |
| RpsN/S14-M | |||||
| Sequence | (M)AKKSMIAKAARKPKFSARGYTRCQICGRPHSVYKFGICRVCLRKMANEGLIPGLKKASW (61aa) | ||||
| 1a | reference isoform LMG 6442 (NCTC 10842) | 6728.11 | ±0.00 | 80.303% (53/66) | |
| 2 | (M)AKKSMIAKAARKPKFSVRGYTRCQICGRPHSVYKDFGICRVCLRKMANEGLIPGLKKASW (61aa) | 6756.16 | +28.05 | 3.030% (2/66) | |
| 3 | (M)AKKSMIAKAARAPKFSSRGYTRCQICGRPHSVYKDFGICRVCLRKMANEGLIPGLKKASW (61aa) | 6687.01 | +41.10 | 16.667% (11/66) | |
| RpmC/L29 | |||||
| Sequence | MKYIDISAKSMSELNALLKEKKVLLFTLRQKLKTMQLTNPNEIGETKKDIARINTAISAAK (61aa) | ||||
| 1a | reference isoform LMG 6442 (NCTC 10842) | 6893.22 | ±0.00 | 47.761% (32/67) | |
| 2 | MKYTEISAKSVSELTALLKEKKVLLFTLRQKLKTMQLTNPNEIRDTKKEIARINTAI SAAK (61aa) | 6949.27 | +56.05 | 19.403% (13/67) | |
| 3a | MKYIDISAKSISELNALLKEKKVLLFTLRQKLKTMQLTNPNEIRDTKKEIARINTAISAAK(61aa) | 6974.32 | +81.10 | 32.836% (22/67) | |
| RplX/L24-M | |||||
| Sequence | (M)AVKYKIKKGDEVKVIAGDDKGKVAKVIAVLPKKGQVIVEGVKVAKKAVKPTEKNPNGGFISKEMPIDISNVAKVEG (77aa) | ||||
| 1a | reference isoform LMG 6442 (NCTC 10842) | 8026.59 | ±0.00 | 47.751% (32/67) | |
| 2 | (M)AIKYKIKKGDEVKVIAGDDKGKVAKVLAVLPKKGQVIVEGVKVAKKAVKPTDKNPNGGFVSKEMPIDISNVAKVEG (77aa) | 8012.56 | –14.03 | 17.910% (12/67) | |
| 3a | (M)AVKYKIKKGDEVKVIAGDDKGKVAKVIAVLPKKGQVIVEGVKVAKKAVKPTDKNPNGGFISKEMPIDISNVAKVEG (77aa) | 8012.56 | –14.03 | 32.836% (22/67) | |
| 4 | (M)AIKYKIKKGDEVKVIAGDDKGKVAKVLAVLPKKGQVIVEGIKVAKKAVKPTDKNPNGGFVSKEMPIDISNVSKVEG (77aa) | 8042.59 | +16.00 | 1.493% (1/67) | |
| RpsT/S20-M | |||||
| Sequence | (M)ANHKSAEKRARQTIKRTERNRFYRTRLKNLTKAVRVAVASGDKDAALVALKDANKNFHSFVSKGFLKKETASRKVSRLAKLVSTLAA (88aa) | ||||
| 1a | reference isoform LMG 6442 (NCTC 10842) | 9741.33 | ±0.00 | 48.529% (33/68) | |
| 2 | (M)ANHKSAEKRARQTIKRTERNRFYRTRLKNLTKAVRVAVANGDKDAALLALKDVNKNFHSFVSKGFLKKETASRKVSRLAKLVSTLAA (88aa) | 9810.43 | +69.10 | 16.176% (11/68) | |
| 3 | (M)ANHKSAEKRARQTIKRTERNRFYRTRLKNLTKAVRVAVANGDKDAALLALKDVNKNFHSFVSKGFLKKKTASRKVSRLAKLVSTLAA (88aa) | 9809.49 | +68.14 | 1.471% (1/68) | |
| 4a | (M)ANHKSAEKRARQTIKRTERNRFYRTRLKNLTKAVRVAVTSGDKDAALLALKDVNKNFHSFVSKGFLKKETASRKVSRLAKLVSTLAA (88aa) | 9813.43 | +72.10 | 32.353% (22/68) | |
| 5 | (M)ANHKSAEKRARQTIKRTERNRFYRTRLKNLTKAVRVAVANGDKDAALLALKDVNKNFHSFVSKGFLKKETASRKVGRLAKLVSTLAA (88aa) | 9780.41 | +39.08 | 1.471% (1/68) | |
| RpsS/S19-M | |||||
| Sequence | (M)ARSLKKGPFVDDHVMKKVLAAKAANDNKPIKTWSRSMIIPEMIGLFNVHNGKGFIPVYVTENHIGYKLGEFAPTRTFKGHKGSQKKIGK (93aa) | ||||
| 1a | reference isoform LMG 6442 (NCTC 10842) | 10277.10 | ±0.00 | 47.761% (32/67) | |
| 2 | (M)ARSLKKGPFVDDHVMKKVLAAKAANDNKPIKTWSRRSTIIPEMIGLTFNVHNGKSFIPVYVTENHIGYKLGEFAPTRTFKGHKGSVQKKIGK (93aa) | 10277.04 | –0.06 | 14.925% (10/67) | |
| 3a | (M)ARSLKKGPFVDDHVMKKVLAAKAANDNKPIKTWSRRSMIIPEMIGLTFNVHNGKSFIPVYVTENHIGYKLGEFAPTRTFKGHKGSVQKKIGK (93aa) | 10307.12 | +30.02 | 32.836% (22/67) | |
| 4 | (M)ARSLKKGPFVDDHVMEKVLAAKATNDNKPIKTWSRRSTIIPEMIGLTFNVHNGKSFIPVYVTENHIGYKLGEFAPTRTFKGHKGSVQKKIGK (93aa) | 10308.00 | +30.90 | 4.478% (3/67) | |
aObserved in test population AA numbering including start-methionine, if mass spectrometry indicates its absence it is written in brackets (M).