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. 2002 Feb;13(2):412–424. doi: 10.1091/mbc.01-09-0434

Figure 2.

Figure 2

OCH1 transcript levels are dependent on SLN1 and SKN7. Representative Northern in which equivalent amounts of mRNA prepared from SLN1+ skn7Δ (JF1904) and sln1* skn7Δ (JF2052) strains carrying various SKN7 plasmids were subjected to electrophoresis. Plasmids were pSL232, SKN7+; pCLM699, skn7D427N; pCLM700, skn7D427E and pRS315, empty vector. The RNA blot was then hybridized with a radiolabeled OCH1 probe and a probe to the SLN1-independent DED1 gene for normalization. Because analysis of OCH1 hybridization patterns in an och1 null mutant (our unpublished results) suggested that both bands of the doublet are due to OCH1 hybridization, both bands were used in quantitation. Normalized expression was calculated by dividing OCH1 values by DED1 values in each lane after phosphoimage analysis. The average increase in OCH1 levels in the sln1-21 strain was 2.4-fold (n = 9). Shown is a single representative autoradiogram.