Table 3.
OCH1-lacZ reporter activity depends on the conserved D427 of the Skn7p receiver and on the DNA binding domain
| Relevant SKN7 genotype | Relative β-galactosidase activity OCH1-lacZ
|
|
|---|---|---|
| SLN1a | sln1*a | |
| SKN7b | 100.0 (8.7) | 368 (38.3) |
| skn7-D427N | 24.4 (2.6) | 23.0 (6.1) |
| skn7-hsfΔ | 14.0 (3.3) | 15.3 (2.6) |
| skn7-S137A, R140A | 10.1 (1.9) | 14.2 (2.7) |
| skn7Δ | 8.5 (1.0) | 11.3 (1.0) |
SLN1 skn7Δ (JF1755) and sln1* skn7Δ (JF1756) strains carrying the OCH1-lacZ reporter (pSL1158) (−355 to +28) were transformed with each of five different plasmids and the β-galactosidase activity measured in cultures from four to six individual transformants from each strain. Values are expressed relative to the SKN7 SLN1 strain, which was set at 100. The actual average value in Miller units of the SKN7 SLN1 strain was 107.9. Values are given with the standard deviation of the mean in parentheses.
SKN7 plasmids were pSL232, SKN7+; pCLM699, skn7D427N; pSL1090, skn7 hsfΔ; and pSL1108, skn7 S137A R140A. The skn7Δ strain carries an empty vector.