Table.

Mutations in mgrB, phoP and phoQ genes among the colistin-resistant Klebsiella pneumoniae

Isolate ID	Source	Colistin MIC (µg/ml)	MLST	Carbapenemase	mgrB	phoP	phoQ	Accession number
Kp1	Blood	16	ST86	Nil	Nil	Alanine 114 arginine (0.22)	Leucine 209 cysteine (0.00*)	bPAW96459.1 cNRQZ01000030.1
Kp2	Blood	32	ST11	Nil	Elongated mgrB of 55 amino acids	Nil	Nil	aPBD34554.1
Kp3	Blood	16	ST14	OXA48-like	Nil	Nil	Tryptophan 161 leucine (0.30)	cMH211110
Kp4	Blood	32	ST14	OXA48, NDM	No amplification	Nil	Tryptophan 161 leucine (0.30)	cMH211111
Kp5	Blood	32	ST14	OXA48-like	Truncated by IS1R of IS1 family	Nil	Nil	aMH590725
Kp6	Blood	8	ST231	OXA48-like	Premature stop codon at 21st amino acid and change in the sequence of protein after 10th amino acid	Nil	Nil	aMH807824
Kp7	Blood	8	ST15	KPC	Truncated by IS903B group of IS5 family	Nil	Nil	aMH590724
Kp8	Blood	16	ST231	OXA48-like	Truncated by ISKpn14 of IS1 family	Nil	Nil	aMH590726
Kp9	CSF	16	ST14	OXA48-like	Truncated by ISKpn26 of IS5 family	Nil	Changes from 218 to 239 and 436 to 448 amino acids	aMH590727 cMH807825
Kp10	Exudate	8	ST23	NDM	Nil	Nil	Glycine 117 aspartic acid (1.00)	cMH211112
Kp11	Exudate	4	ST2096	OXA48-like	Nil	Nil	Valine 370 glutamic acid (0.03*)	cMH211113
Kp12	Exudate	32	ST14	OXA48, NDM	Cysteine to glycine at 28th amino acid (0.00*)	Threonine 151 Alanine (0.00*)	Nil	aMH337365 bMH211116
Kp13	Exudate	16	ST147	OXA48, NDM	Nil	Glutamic acid 22 lysine (0.49)	Nil	bMH211117
Kp14	Exudate	32	ST231	OXA48-like	Premature stop codon at 21st amino acid and change in the sequence of protein after 10th amino acid	Nil	Nil	aMH807823
Kp15	Respiratory	32	ST14	NDM	Cysteine to glycine at 28th amino acid (0.00*)	Nil	Leucine 172 glutamine (0.01*); Tryptophan 182 serine (0.04*)	aMH337366 cMH211114
Kp16	Respiratory	8	ST231	OXA48-like	Nil	Nil	Valine444^, phenylalanine 445 glycine (0.00*)	cMH211115
Kp17	Respiratory	8	ST147	OXA48-like	Methionine to arginine first amino acid (0.00*)	Nil	Nil	aMH590728
Kp18	Respiratory	16	ST231	OXA48-like	Premature stop codon at 21st amino acid and change in the sequence of protein after 10th amino acid	Nil	Nil	aMH337364
Kp19	Respiratory	8	ST2957	KPC-9	No mutation; associated with IS102 of IS5 family	Nil	Proline 424 leucine (0.37) V446W (0.00*), change from 448th amino acid	aPAT25546.1 cCJ307_16160

^amgrB accession number; ^bphoP accession number; ^cphoQ accession number. Numbers in parenthesis indicate the SIFT score; values with ^*<0.05 show that the mutation affects the protein function. ^#Position of insertion in mgrB gene. ^{^}Indicates Valine deletion at 444^th position in phoQ. MLST, multilocus sequence typing; SIFT, sorting intolerant from tolerant; MIC, minimum inhibitory concentration; IS, insertion sequence