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. 2019 Feb;149(2):270–275. doi: 10.4103/ijmr.IJMR_2055_17

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Copyright: © 2019 Indian Journal of Medical Research

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Fig. 2 — Southern hybridization with undigested plasmids of Pediococcus pentosaceus isolates against erm(B) gene probes. (A) Plasmids; (B) Southern blot analysis of plasmids hybridized with internal segments of erm(B) gene obtained by PCR and labelled with digoxigenin. Lane M: HindIII digested λ-DNA marker; lanes 1 to 15: P. pentosaceus isolates IB4-2A, IB4-2B, IB3-3, 8, B-1, IB3-1A, IB3-1B, IB3-2, IB6-2A, DB3-E4, DB3-STR3B, CHS-3E, 3, 4, IB2-1, respectively. The isolates IB4-2A and IB6-2A in lanes 1 and 8 respectively were positive for erm(B). The same isolates were also found positive for msr(C) with similar localization (data not shown). The localization of these genes was found on high molecular weight plasmids.