Table 3.
Analysis of variance (ANOVA) of Aspergillus section Flavi (AsF) and aflatoxin B1 (AFB1) contamination in the 2 years (2016 and 2017), different sampling times (14, 28, 42, and 56 days after inoculation-DAI) and treatments considered in the study (untreated, single and co-inoculum with Fusarium verticillioides and Fusarium graminearum).
| Incidence of AsF (%) | Aflatoxin B1 (μg/kg) | |||||
|---|---|---|---|---|---|---|
| Year (A) | ∗∗ | Std Dev | ∗∗ | Std Dev | ||
| 2016 | 19.9 | B | 8.3 | 150.8 | B | 13.8 |
| 2017 | 29.4 | A | 8.9 | 348.0 | A | 11.8 |
| Sampling | n.s. | ∗∗ | ||||
| time (B) | ||||||
| 14 DAI | 18.6 | 8.5 | 36.5 | C | 7.9 | |
| 28 DAI | 24.8 | 9.5 | 70.4 | B | 8.0 | |
| 42 DAI | 26.3 | 9.2 | 278.8 | A | 13.3 | |
| 56 DAI | 26.8 | 8.1 | 510.2 | A | 13.9 | |
| Thesis (C) | ∗∗ | ∗∗ | ||||
| Untreated | 6.2 | B | 1.9 | 1.5 | C | 2.3 |
| A. flavus | 32.9 | A | 8.4 | 319.7 | B | 13.4 |
| A. flavus + F. verticillioides | 23.1 | A | 4.5 | 236.6 | B | 9.6 |
| A. flavus + F. graminearum | 34.3 | A | 11.3 | 377.5 | A | 8.5 |
| AxB | ∗ | ∗ | ||||
| BxC | n.s. | ∗∗ | ||||
| AxC | n.s. | ∗ | ||||
| AxBxC | n.s. | ∗ | ||||
n.s., not significative; ∗P ≤ 0.05; ∗∗P ≤ 0.01; Std Dev, standard deviation.