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. 2019 Jun 6;6:31. doi: 10.3389/fsurg.2019.00031

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Copyright © 2019 Valdes, Juvekar, Agar, Gioux and Golby.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Real time, quantitative fluorescence imaging system coupled with single snapshot optical properties imaging. (A) Schematics of a qFI system that performs simultaneous SSOP imaging and fluorescence detection by acquiring simultaneous reflectance and fluorescence imaging under SSOP mode. (B) Data processing scheme in which reflectance images are processed under SSOP conditions to estimate the absorption μ_a and reduced scattering μ_s’. (C) A light transport model is used to correct the raw fluorescence for the effects of tissue optical properties to calculate the quantitative fluorescence. Bottom panel shows one frame of a real-time dynamic video of tissue simulating phantoms displaying the raw fluorescence (uncorrected), the empirically corrected fluorescence (F/R), and the quantitative fluorescence using SSOP (qF-SSOP). Figure adapted with permission from Valdes et al. (37).