FIGURE 4.

Altered responses to UPS inhibitors and cycloheximide between heterozygous knockout and wild-type adult cardiomyocytes. (A) Representative Western blotting of cMLCK and total MLC2v using adult cardiomyocytes isolated from Mylk3^wild/wild and Mylk3^wild/- mice at 4 months of age at time 0 and 48 h of incubation with or without UPS inhibitors. The cropped blots are used in the figure. (B) Fold difference of cMLCK relative to total MLC2v with the value of Mylk3^wild/wild at time 0 defined as 1 (n = 4 each). (C) Representative immunostaining of cMLCK and MLC2v in the heart sections (n = 3 each). (D) Representative immunostaining of cMLCK and Z-disk protein α-actinin2, and cMLCK and M-line protein myomesin in the heart sections. Data are expressed as mean ± SEM. ^∗P < 0.05. Bars = 20 μm.