Table 1.
Conversion and enantioselectivity of AMDase variants towards methylvinylmalonic acid derivatives 1.[a]
| |||||
|---|---|---|---|---|---|
| Entry | Substrate | Variant | Time [h] |
Conversion [%] |
ee
[%] |
| 1 | 1 a | IPLL | 1 | >99 | 97 (R) |
| 2 | 1 a | CLGIPL | 3 | >99 | 66 (S) |
| 3 | 1 b | IPLL | 1 | >99 | >99 (R) |
| 4 | 1 b | CLGIPL | 3 | 98 | 98 (S) |
| 5 | 1 c | IPLL | 3 | 90 | >99 (R) |
| 6 | 1 c | CLGIPL | 3 | 85 | >99 (S) |
| 7 | 1 d | IPLL | 3 | 98 | >99 (R) |
| 8 | 1 d | CLGIPL | 18 | >99 | >99 (S) |
[a] Reaction conditions: Tris‐HCl buffer (50 mm, pH 8.0 at 30 °C), substrate 1 (10 mm), 10 % (v/v) or 50 % (v/v) of crude cell extract including IPLL or CLGIPL, respectively. Reaction temperature: 30 °C.