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. Author manuscript; available in PMC: 2019 Jun 13.
Published in final edited form as: Science. 2019 Mar 7;363(6431):1098–1103. doi: 10.1126/science.aau5721

Fig. 2. Activation time distribution of SOS.

Fig. 2.

(A and B) Histograms of the activation time and rejection time of Grb2-mediated SOSFL recruitment from the single-molecule activation assay. The solid line is fitting to the model in (C); the fitted values are kN = 0.02 s−1 and k−1 = 0.016 s−1. The dashed line represents the prediction by the model using fitted values from the activation time distribution. (C) A simple model of SOSFL activation. kN denotes the transition rate constants for the kinetic intermediates, and k−1 represents the dissociation rate constants from membranes. SOSsoln, SOS in solution. (D) Without a kinetic intermediate (N = 0), the activation time distribution is an exponential distribution peaked at t = 0. In contrast, the existence of at least one intermediate produces the characteristic rise-and-decay shape for the activation time distribution.