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. 2019 Jun 6;10:1285. doi: 10.3389/fimmu.2019.01285

Figure 5.

Figure 5

Chloroquine but not rapamycin attenuates IL-1β release from monocyte-derived M-CSF-differentiated human macrophages. (A,B) Macrophages were stimulated with 10 ng/mL LPS for times as indicated and pro-IL-1β was quantified with western blot. (C–E) Macrophages were incubated with 10 ng/mL LPS for 5 h with or without 500 nM rapamycin or 20 μM chloroquine and pro-IL-β and ASC quantified with western blot (n = 3). Columns are mean with SEM. (F,G) Macrophages were incubated with 10 ng/mL LPS for 5 h with or without 500 nM rapamycin or 20 μM chloroquine phosphate, then 3 mM ATP for 30 min. IL-1β and TNF release were quantified in conditioned medium with ELISA and percent change in output calculated. Data are normalized to control = 100%. (H,I) Macrophages were incubated with 10 ng/mL LPS for 5 h with or without 500 nM rapamycin or 20 μM chloroquine phosphate. IL-1β and TNF mRNA was quantified with PCR. All columns are mean with SEM (n = 3–6). *p < 0.05, **p < 0.01 as compared to control or as indicated (paired student t-test).