Table 1.
Antibodies used.
| Antibody | Source/company | Application |
|---|---|---|
| Anti-NEK7 (Ab133514) | Abcam | Western blot |
| Anti-NEK7 (bs7758R-A488) | Bioss | Confocal microscopy |
| Anti-NLRP3 (D2P5E) | Cell signaling | Western blot |
| Anti-ASC (D2W8U) | Cell signaling | Western blot |
| Anti-ASC (bs6741R-A647) | Bioss | Confocal microscopy |
| Anti-procaspase-1 (Ab1872) | Abcam | Western blot |
| Anti-pro-IL-1β (AF-401-NA) | R&D | Western blot |
| Anti-SDHA (complex II) (Ab14715) | Abcam | Western blot |
| Anti mTOR (7C10) | Cell signaling | Western blot |
| Anti phospho-mTOR (Ser2448) (D9C2) | Cell signaling | Western blot |
| Anti p70 S6 kinase (#9202) | Cell signaling | Western blot |
| Anti phospho-p70 S6 kinase (Thr389) (#9205) | Cell signaling | Western blot |
| Anti ubiquitin (P4D1) | Cell signaling | Western blot |
| Anti LC3B (D11) (#3868) | Cell Signaling | Immune fluorescence microscopy |
| Anti-rabbit IgG HRP (7074S) | Cell signaling | Western blot |
| Anti-mouse IgG HRP (7076S) | Cell signaling | Western blot |
| Anti-goat IgG HRP (sc-2020) | Santa cruz | Western blot |
| Anti-cleaved Caspase-8 (Asp387)(#9429) | Cell signaling | Western blot |