Table 1.
S. pombe strains used in this study
| P1 | mcm4ts(cdc21-M68) leu1-32 h− | Nasmyth and Nurse, 1981 |
| P886 | mcm4+(cdc21+)-GFP5∷kanr nmt1(41X)-cdc13∷LEU2 cig1Δ∷ura4+ cig2Δ∷ura4+ cdc13Δ∷ura4+ ura4-Δ18 leu1-32 ade6-M210 h− | Derived from Fisher and Nurse (1995) |
| P900 | mcm4+(cdc21+)-GFP5∷kanr cyr1Δ∷LEU2 sxa2Δ∷ura4+ leu1-32 ura4-D18 h− | Derived from Stern and Nurse (1997) |
| P937 | pat1-114 h− | |
| P958 | mcm4+(cdc21)-GFP5∷kanr pat1-114 h− | |
| P978 | mcm4+(cdc21)-GFP5∷kanr//mcm4+(cdc21)-GFP5∷kanr pat1-114//pat1-114 ade6-M210//ade6-M216 leu1-32//+ h−//h− | |
| P1025 | mcm6+-GFP5∷kanr pat1-114 h− | |
| P1023 | mcm4ts(cdc21-M68)-td∷ura4+ leu1-32 ura4-D18 h− | |
| P1027 | mcm2+-GFP5∷kanr pat1-114 h− | |
| P1037 | mcm4ts(cdc21-M68)-td∷ura4+ pat1-114 h− | |
| P1039 | mcm4ts(cdc21-M68) pat1-114 h+ | |
| P1096 | mcm2+-GFP5∷kanr mcm4ts(cdc21-M68)-td∷ura4+ pat1-114 |