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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Nat Chem Biol. 2019 Apr 1;15(5):433–436. doi: 10.1038/s41589-019-0256-z

Figure 2. Non-invasive calcium imaging in the liver of a living mice with Orange CaMBI.

Figure 2.

(a) A mouse transgenic for a floxed-stop Orange CaMBI 110 gene was injected with adeno-associated virus expressing Cre recombinase to activate gene expression in the liver. Raw bioluminescence images and overlay of pseudocolored bioluminescence signal over a bright-field image at the indicated time points t1 through t4. Color bar, linear lookup table relating pseudocolor to raw counts per pixel. Scale bar, 1 cm. (b) Time-course of bioluminescence of Orange CaMBI 110 from different lobes of mouse liver. Interspersed 2-s exposures of luminescence and bright field were acquired every 6 s. Integrated luminescence signal from each lobe normalized to its value at 150 s after substrate injection, when baseline luminescence stabilized, is shown. Normalization to a fitted second-order polynomial function was performed to correct for signal decay.