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(A-B) The characteristic fold that separates the pylorus and ileum, as well as the primary region of the pylorus is lost when Wg is tethered. (C-D’) The majority of the tightly-packed pylorus cells are lost in NRT-Wg mutants. wild-type Wg: wg{KO, Wg-HA}%; tethered Wg: wg{KO, NRT-Wg-HA}%. Orange arrow marks the MHB. Silver arrow marks the fold between the pylorus and ileum. Anterior, left. Scale bars: (A-B) 50 μm, (C-D’) 25 μm.
