Fig 5. Inhibition of Wingless activity phenocopies the epithelial defects resulting from the tethering of Wg.

(A-B’) The transition zone at the MHB is lost upon inhibition of Wg by overexpression of notum. Tightly clustered cells with condensed nuclei are detected in both midgut and hindgut. (C-D’) Fas III expression is no longer restricted to the hindgut, replaced by expression both in the midgut and the hindgut. In addition, the fold between the pylorus and ileum is no longer evident. Orange arrow marks the MHB. Green arrow marks the fold between pylorus and ileum. Anterior, left. Scale bars: (A, B, C, D) 50 μm, (A’, B’, C’, D’) 25 μm.