Figure 5.
(A) Low molecular weight RNAs from silenced and nonsilenced strains. Low molecular weight RNA was isolated from the strains indicated, blotted, and hybridized with a 32P-labeled β-gal sense probe. The position of ∼23mers is indicated by an arrowhead. (B) 32P-Labeled dsRNA was generated by in vitro transcription of a PSV-A gene fragment and incubated with a partially purified dsRNase preparation from Dictyostelium. Reaction products were separated by PAGE in parallel with small RNA isolated from RNAi-silenced β-gal cells. RNA was blotted and hybridized with a 32P-labeled β-gal probe. The blot was exposed for 1 wk (left) and over night (right).