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. 2002 Feb;13(2):445–453. doi: 10.1091/mbc.01-04-0211

Figure 5.

Figure 5

(A) Low molecular weight RNAs from silenced and nonsilenced strains. Low molecular weight RNA was isolated from the strains indicated, blotted, and hybridized with a 32P-labeled β-gal sense probe. The position of ∼23mers is indicated by an arrowhead. (B) 32P-Labeled dsRNA was generated by in vitro transcription of a PSV-A gene fragment and incubated with a partially purified dsRNase preparation from Dictyostelium. Reaction products were separated by PAGE in parallel with small RNA isolated from RNAi-silenced β-gal cells. RNA was blotted and hybridized with a 32P-labeled β-gal probe. The blot was exposed for 1 wk (left) and over night (right).

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