Table 1.
Silencing efficiency in wild type and RdRP mutant cells
Host strain transformation | AX2
|
RrpA−
|
DosA−
|
|||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
β-Gal + control
|
β-Gal +
mcRNAi
|
β-Gal + control
|
β-Gal +
mcRNAi
|
β-Gal + control
|
β-Gal +
mcRNAi
|
|||||||
Clone 1A | 128 | Clone 2A | 6,1 | Clone 3A | 378 | Clone 4A | 10,2 | Clone 5A | 1257 | Clone 6A | 4,4 | |
Clone 1B | 383 | Clone 2B | 8,9 | Clone 3B | 255 | Clone 4B | 74 | Clone 5B | 90 | Clone 6B | 8,8 | |
Clone 1C | 457 | Clone 2C | 1,0 | Clone 3C | 23,2 | Clone 4C | 24,3 | Clone 5C | 1024 | Clone 6C | 14,9 | |
Clone 1D | 155 | Clone 2D | 0,6 | Clone 3D | 246 | Clone 4D | 343 | Clone 5D | 233 | Clone 6D | 3,6 | |
Clone 1E | 293 | Clone 3E | 26 | Clone 4E | 89 | Clone 5E | 838 | Clone 6E | 1,8 | |||
Clone 3F | 334 | Clone 4F | 163 | Clone 5F | 413 | Clone 6F | 22,6 | |||||
Clone 3G | 83 | Clone 4G | 656 | Clone 5G | 1184 | Clone 6G | 5,3 | |||||
Clone 3H | 319 | Clone 4H | 16,4 | Clone 5H | 1271 | Clone 6H | 1,11 | |||||
Clone 3I | 24 | Clone 4I | 140 | Clone 5I | 1063 | Clone 6I | 23,0 | |||||
Clone 3J | 200 | Clone 4J | 509 | Clone 5J | 663 | Clone 6J | 4,6 | |||||
Population | 316 | Population | 8,2 | Population | 210 | Population | 253 | Population | 419 | Population | 15,1 | |
Average β-gal activity (U/mg) clones + pop. | 289 ± 128 | 5 ± 4 | 191 ± 132 | 207 ± 214 | 769 ± 428 | 9, 6 ± 8 | ||||||
Unpaired t test p value | 0,0008 | 0,83 | 0,0001 | |||||||||
Average reduction by RNAi | 98% | 0% | 99% |