Skip to main content
. 2019 Jun 13;14(6):e0218330. doi: 10.1371/journal.pone.0218330

Fig 3. Western blotting analysis with novel anti-CD26 mAbs.

Fig 3

Whole cell lysates of MSTO parent, MSTO-CD26, JMN CD26-shRNA, JMN ctrl-shRNA, A549, Jurkat parent or Jurkat-CD26 cells were separated by SDS-PAGE (each, 20 μg), and CD26 was detected by immunoblotting with purified mouse anti-human CD26 mAb (19–32 (i), U16-3 (ii), U38-8 (iii)), or purified goat anti-human CD26 pAb (R&D Systems (iv)). The same blots were stripped and reprobed with antibodies specific for β-actin as a loading control. Data shown are representative of three independent experiments, and similar results were obtained in each experiment.