Fig 8. Germ cell apoptosis is p53 independent and partially regulated by lin-35/Rb during ARD.

(A-B) cep-1(gk38);ced-1::gfp, lin-35(n745);ced-1::gfp and ced-1::gfp animals were used to visualize the number of germ cell corpses per gonad arm (see Materials and methods) for 5 days in control (solid lines) and starvation conditions (dotted lines). The average number of germ cell corpses per gonad arm is shown with the SEM. (C-D) DAPI staining was used to quantify the number of germ cells per gonad arm (see Materials and methods) for 5 days in control (solid lines) and starvation conditions (dotted lines). The average number of germ cells per gonad arm is shown with the SEM. (E-F) The percentage of germ cell corpses among the total germ cells per gonad arm in fed animals (solid lines) and during starvation (red lines) conditions was calculated.