Table 1. Fertility assays in diverse genetic backgrounds used in this study.
| Genotype | Brood Size | Dead Embryos | N | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| WT | 231.8 | ± | 2.3 | 2.1 | ± | 0.1 | 144 | |||
| Recovered WT | 67.0 | ± | 2.9 | *** | 3.8 | ± | 0.3 | *** | 69 | |
| Control WT | 424.9 | ± | 8.3 | 2.6 | ± | 0.3 | 22 | |||
| x WT males | ||||||||||
| Recovered WT | 344.8 | ± | 26.3 | n.s | 2.8 | ± | 0.6 | n.s. | 17 | |
| x WT males | ||||||||||
| Control fog-2(q71) | 324.5 | ± | 6.5 | 2.4 | ± | 0.3 | 46 | |||
| x WT males | ||||||||||
| Recovered fog-2(q71) | 183.2 | ± | 5.6 | * | 4.0 | ± | 0.3 | * | 42 | |
| x WT males | ||||||||||
| Prolonged diakinesis fog-2(q71) | 55.8 | ± | 2.3 | * | 4.8 | ± | 0.2 | * | 51 | |
| x WT males | ||||||||||
| Control fog-1(q253) | 302.3 | ± | 7.0 | 2.5 | ± | 0.1 | 31 | |||
| x WT males | ||||||||||
| Recovered fog-1(q253) | 177.1 | ± | 3.3 | * | 4.4 | ± | 0.2 | * | 35 | |
| x WT males | ||||||||||
| Prolonged diakinesis fog-1(q253) | 65.0 | ± | 1.6 | * | 5.6 | ± | 0.1 | * | 39 | |
| x WT males | ||||||||||
| Control ced-3(n717) | 141.0 | ± | 2.6 | 8.1 | ± | 0.7 | 43 | |||
| Recovered ced-3(n717) | 53.1 | ± | 1.8 | * | 7.3 | ± | 0.5 | * | 41 | |
| Control ced-3(n1286) | 143.8 | ± | 3.2 | 7.5 | ± | 0.4 | 88 | |||
| Recovered ced-3(n1286) | 52.0 | ± | 2.3 | * | 12.3 | ± | 0.8 | * | 58 | |
| Control ced-3(n717) | 315.6 | ± | 8.6 | 19.8 | ± | 3.0 | 11 | |||
| x WT males | ||||||||||
| Recovered ced-3(n717) | 248.9 | ± | 12.3 | ** | 19.3 | ± | 1.9 | n.s. | 20 | |
| x WT males |
Hermaphrodites with the different genetic backgrounds were individually selected at the mid-L4 stage and transferred to new plates every 24 h until they ceased laying embryos. Recovered wild-type hermaphrodites were selected at the mid-L4 stage, starved for 5 days and transferred to new plates every 24 h until they ceased laying embryos. t-test (control vs. recovered). Control fog-1(q253) and fog-2(q71) animals were individually selected in the mid-L4 stage, mated with well-fed males and transferred to fresh plates daily until they ceased laying embryos. Recovered virgin fog-1(q253) and fog-2(q71) animals were selected in the mid-L4 stage and starved for 5 days, then individually refed for 1 day, mated with well-fed males and transferred to fresh plates daily until they ceased laying embryos. Virgin fog-1(q253) and fog-2(q71) animals were selected in the mid-L4 stage and placed on food for 6 days, then mated with well-fed males and transferred to fresh plates daily until they ceased laying embryos. Plates were scored for dead embryos and total progeny. Embryos that did not hatch within 24 h after being laid were scored as dead. Dunn’s test (wild-type values as control). n.s. non significant.
* P ≤ 0.05
** P ≤ 0.01
*** P≤ 0.001