Table 2. Germ cell quantification per gonad arm in different genetic backgrounds under control and starvation conditions.

										5 Days post mid-L4
		mid-L4			N		Control			N		Starvation			N
	WT	141.9	±	1.8	49		468.2	±	2.0	38	**	36.0	±	0.5	54	**
20°C
	fog-2(q71)	143.1	±	2.0	42		315.9	±	2.6	47	**	141.0	±	1.5	48	**
	WT	144.7	±	2.2	30		386.9	±	1.6	20	**	35.5	±	0.8	24	**
15°C
	fog-1(q253)	143.5	±	1.8	30		386.2	±	1.6	30	**	36.0	±	0.6	25	**
	WT	135.3	±	1.5	20		388.5	±	1.8	25	**	34.2	±	0.7	25	**
25°C
	fog-1(q253)	133.5	±	0.8	30		338.5	±	1.8	30	**	141.8	±	1.6	25	n.s.

The gonads of animals under control conditions or starvation were dissected, stained and scored for the number of germ cells using epifluorescence microscopy. Control animals remained on NGM plates seeded with OP50. For starvation conditions, animals were grown on food from L1 to the mid-L4 larval stage and then starved for 5 days. Animals were subsequently picked and dissected. The dissected gonads were stained with DAPI and the number of germ cells per gonad arm was scored under fluorescence microscopy.