Table 5. The number of germ cells per gonad arm under control and starvation conditions.

	ced-1::gfp
Days post mid-L4	Control			N	Starvation			N
0	172.2	±	4.57	45	172.2	±	4.57	45
1	359.6	±	21.88	15	138.4	±	4.72	42
2	406.1	±	21.23	15	89.4	±	3.04	30
3	487.3	±	20.82	17	56.0	±	1.83	31
4	506.8	±	10.94	15	48.4	±	3.02	27
5	505.1	±	19.53	18	43.6	±	1.37	35
6	521.3	±	14.82	18	37.2	±	1.18	30
7	509.6	±	11.76	16	37.8	±	0.75	24
8	545.9	±	13.44	17	37.8	±	1.04	38
9	548.6	±	8.19	17	38.4	±	1.19	34
10	574.7	±	6.56	15	38.4	±	1.19	38

The gonads of animals under control conditions and starvation were dissected, stained and scored for the number of germ cells using epifluorescence microscopy. Control animals were placed in NGM plates seeded with OP50 and transferred to fresh plates daily until they ceased laying eggs. For starvation conditions, animals were placed in unseeded NGM plates and transferred to fresh plates daily, similar to the control animals. Animals were picked and dissected. The dissected gonads were stained with DAPI and the number of germ cells per gonad arm was scored under fluorescence microscopy.