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. 2002 Feb;13(2):454–468. doi: 10.1091/mbc.01-11-0553

Figure 6.

Figure 6

XCL100 is a labile protein in oocytes. (A) stage VI oocytes were microinjected with mRNA (46 ng) encoding Myc-tagged XCL100. Seven hours later, microinjected oocytes were split into three pools and transferred to OR2 containing 50 μg/ml cycloheximide (CHX) alone, CHX plus 5 μg/ml progesterone, or CHX plus 0.2 M sorbitol. Samples were collected at the indicated times and immunoblotted with 9E10 (to detect XCL100), DC3 (to detect p42 MAPK), or the MEK antiserum 662. The first two lanes of each blot represent uninjected oocytes treated with (M) or without (G2) 5 μg/ml progesterone as controls for active and inactive p42 MAPK, respectively. (B) XCL100 proteins were quantified using the Kodak Image Station 440CF and 1D Image Analysis Software. Data are shown as mean values ± SEM for two independent experiments.