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. 2002 Feb;13(2):469–479. doi: 10.1091/mbc.01-07-0356

Figure 6.

Figure 6

SEPA::GFP and actin colocalize in ring structures and at the hyphal tip. (A-D) Strain AKS70 (sepA::gfp) was stained with anti-actin antibodies, Calcofluor, and Hoechst 33258 to visualize actin, septa, and nuclei. Images are ordered by their stage of septation from earliest to latest. (A) SEPA::GFP and actin rings are visible, but no septal material is present. (B) SEPA::GFP and actin rings have started to constrict and a faint septal ring is present (arrowheads). The arrow indicates a mature septum, which is brighter than the septal ring; SEPA::GFP and actin are absent at this stage. (C and D) SEPA::GFP and actin colocalize during constriction, appearing as hourglass shapes (see text). (E) SEPA::GFP and actin colocalize at the very tips of hyphae in strain AKS70. (F) Control showing that fluorescence due to actin staining is not visible using the fluorescein isothiocyanate filter used for GFP. Also shown are actin, septa, and nuclei in wild-type strain A28, which lacks SEPA::GFP. Bars, 3 μm.