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. 2002 Feb;13(2):503–514. doi: 10.1091/mbc.01-07-0365

Figure 2.

Figure 2

pag1-1 is defective in cell shape and mating projection formation. (A) Wild-type diploid cells (NY1523) and pag1-1 diploid cells (NY2342) growing exponentially in YPD at 30°C were fixed and stained with fluorescently labeled phalloidin. Cells were imaged in differential interference contrast (DIC) mode and fluorescence mode, respectively. Bar, 4 μm. (B) pag1-1 diploid cells are significantly rounder than wild-type cells. From the DIC images, we measured the lengths of the major axis and minor axis of budded cells of the two strains shown in A. Distributions of the major axis/minor axis ratio are plotted as a histogram. (C) Wild-type MATa cells (NY13) and pag1-1 MATa cells (NY2333) grown in YPD at 30°C were treated with α-factor (5 μg/ml) at 30°C for 3 h then fixed and stained with fluorescently labeled phalloidin. Bar, 4 μm.