Time course of milk fatty acid yield of de novo (<16 carbons; Panel A), mixed source (16 carbons; Panel B), preformed (>16 carbons; Panel C) origin and trans-10, C18:1 (Panel D) of cows fed sodium bicarbonate at 3.2% of diet DM (control; NaHCO), sodium acetate at 2.9% of diet DM (NaAc), and calcium butyrate at 2.5% of diet DM (CaBu; equal carbon mass of NaAc), (n = 12 per treatment). Least-square means and standard errors are shown and means that do not share a superscript differ within timepoint (P < 0.05).