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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: J Dairy Sci. 2019 Apr 10;102(6):5172–5181. doi: 10.3168/jds.2018-16024

Table 1.

Ingredient and nutrient composition of experimental diet comparing sodium acetate to sodium bicarbonate DCAD control and equivalent carbon mass of butyrate as calcium butyrate1.

Treatments2
Control NaAc3 CaBu4

Ingredients, % DM
 Corn silage 35.9 36.0 36.2
 Alfalfa haylage 12.9 13.0 13.0
 Cracked corn 10.9 11.0 11.0
 Canola meal 10.5 10.6 10.6
 Bakery byproduct 4.68 4.70 4.72
 Roasted soybeans 4.68 4.70 4.72
 Molasses 4.68 4.70 4.72
 Whole cottonseed 4.29 4.30 4.32
 Grass hay 3.90 3.91 3.93
 Ground corn 2.34 2.35 2.36
 Mineral-vitamin mix5 1.61 1.61 1.61
 NPN6 0.39 0.39 0.39
 Sodium bicarbonate 3.21
 Sodium acetate 2.87
 Calcium butyrate 2.47
Nutrient composition, % DM
 Crude protein 14.6 ± 0.7 14.6 ± 0.7 14.7 ± 0.7
 Neutral detergent fiber 30.7 ± 0.7 30.8 ± 0.7 30.9 ± 0.7
 Acid detergent fiber 12.5 ± 0.1 12.5 ± 0.1 12.6 ± 0.1
 Starch 18.7 ± 0.9 18.7 ± 0.9 18.8 ± 0.9
 Fatty acids 4.31 ± 0.6 4.33 ± 0.6 4.35 ± 0.6
Fatty acids, % of FA
 C16:0 7.40 ± 1 7.43 ± 1 7.46 ± 1
 C18:0 2.87 ± 0.2 2.88 ± 0.2 2.89 ± 0.2
 C18:1, n-9 10.2 ± 1.5 10.2 ± 1.5 10.2 ± 1.5
 C18:2, n-6 18.8 ± 2.6 18.8 ± 2.6 18.9 ± 2.6
 C18:3, n-3 2.46 ± 0.09 2.47 ± 0.09 2.48 ± 0.09
DCAD, mEq/100 g 5.22± 0.17 4.78 ± 0.11 1.24 ± 0.002
1

Cows were fed the same basal TMR diet (1.67 Mcal / kg NEL) and treatments were mixed into the basal diet.

2

Treatments were sodium bicarbonate control (3.2% of diet DM), sodium acetate at 2.9% of diet DM (targeting 10 mol of acetate/d; NaAc; Food grade anhydrous sodium acetate, Kemin Industries, Inc., Des Moines, IA), calcium butyrate at 2.5% of diet DM (CaBu; Kemin Industries Inc.) to provide an equivalent carbon mass as NaAC treatment.

3

Acetate contained in NaAc provided 2.09 Mcal NEL/d

4

Butyrate contained in CaBu provided 2.65 Mcal NEL/d

5

Contained (%, as fed basis): 45.8 dried corn distillers grains with solubles; 35.8 limestone (38% Ca); 8.3 magnesium oxide (54% Mg); 6.4 salt; 1.73 vitamin ADE premix; 1.09 selenium premix (0.06% selenium); and 0.88 trace mineral mix.

6

Non protein nitrogen fed as a slow release urea (Optigen, Alltech Inc., Lexington, KY; 259% CP, DM basis).