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. Author manuscript; available in PMC: 2019 Jun 13.
Published in final edited form as: J Dairy Sci. 2018 Oct 11;101(12):10911–10921. doi: 10.3168/jds.2018-15071

Table 3.

Effect of diet composition investigated in 3 experiments on the rumen pool size before and after feeding determined by rumen evacuation

Experiment Prefeeding Postfeeding SEM P-value Time1
1: Fiber2
 Rumen pool, kg
  DM 19.9 25.5 1.5 <0.001
  NDF 13.6 16.5 1.0 <0.001
  iNDF1203 7.9 8.5 0.6 0.02
  ADF 7.2 8.5 0.5 <0.001
  Starch 2.2 3.4 0.2 <0.001
  Lignin 1.5 1.7 0.1 <0.001
 Rumen composition, %
  NDF 69.5 64.7 0.9 <0.001
  iNDF120 39.6 33.7 0.7 <0.001
  ADF 36.5 33.4 0.4 <0.001
  Starch 10.9 13.3 0.4 <0.001
  Lignin 7.5 6.8 0.1 <0.001
2: Starch4
 Rumen pool, kg
  DM 17.5 23.4 1.0 <0.001
  NDF 10.3 12.4 0.7 <0.001
  iNDF120 6.3 7.2 0.4 <0.001
  Starch 0.9 2.0 0.1 <0.001
 Rumen composition, %
  NDF 58.9 52.8 1.3 <0.001
  iNDF120 36.3 30.8 1.1 <0.001
  Starch 4.7 8.4 0.3 <0.001
3: Fatty acids5 4.7 8.4 0.3 <0.001
 Rumen pool, kg
  DM 12.2 15.6 0.53 <0.001
  NDF 6.8 7.9 0.25 <0.001
  iNDF2406 3.5 3.9 0.15 0.02
  ADF 4.5 5.2 0.17 <0.001
  Starch 0.6 1.5 0.08 <0.001
  Lignin 1.1 1.2 0.05 <0.001
 Rumen composition, %
  NDF 57.0 51.2 0.41 <0.001
  iNDF240 29.6 25.1 0.82 <0.001
  ADF 37.6 33.8 0.45 <0.001
  Starch 4.8 9.1 0.37 <0.001
  Lignin 8.9 7.8 0.23 <0.001
1

Treatment effects have been previously published in Oba and Allen (2000) for experiment 1, Oba and Allen (2003) for experiment 2, and Harvatine and Allen (2006) for experiment 3. No treatment by time interactions occurred for any responses (P > 0.05).

2

A 2 × 2 factorial design of brown midrib (BMR) and isogenic conventional (CONV) corn silage in a high- (38%) or low-NDF (29%) diet. Rumen evacuations were preformed 2 h before and 2 h after feeding at 1200 h.

3

iNDF120 = indigestible NDF, estimated as fiber remaining after 120 h of in vitro ruminal fermentation.

4

A 2 × 2 factorial design of dry ground (DG) and high-moisture ground (HM) corn in a high- (32%) and low-starch (21%) diet. Rumen evacuations were preformed 4 h before and 4 h after feeding at 1400 h.

5

CON = no-supplement fat control; SFA = prilled saturated fatty acid supplement (Energy Booster-100, Milk Specialties Inc., Eden Prairie, MN) at 2.5% of diet DM; UFA = calcium salts of unsaturated fatty acids (Megalac-R, Church and Dwight Co. Inc., Ewing, NJ) at 2.5% of diet DM; MIX = 50:50 mixture of SFA and UFA at 2.5% of diet DM. Rumen evacuations were preformed 2 h before and 4.5 h after feeding at 0900 h.

6

Indigestible NDF, estimated as fiber remaining after 240 h of in vitro ruminal fermentation.