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. 2002 Feb;13(2):542–557. doi: 10.1091/mbc.01-10-0476

Figure 5.

Figure 5

Inactivation of SJL2-encoded PI 5-Pase activity affects α-factor maturation. Wild-type (WT; SEY6210; lane 1), sjl3Δ (JGY131; lane 2), sjl2Δ sjl3Δ (JGY132; lane 3), SJL2 sjl3Δ (JGY132 carrying pRS416SJL2; lane 4), sjl2C446S sjl3Δ (JGY132 carrying pRS416sjl2C446S; lane 5), and sjl2D850S sjl3Δ (JGY132 carrying pRS416sjl2D850S; lane 6) cells were metabolically labeled at 26°C for 45 min, and secreted α-factor was immunoprecipitated from the culture supernatants. Samples were analyzed by SDS-PAGE and fluorography. Precursor levels of α-factor were quantified by phosphoimage analysis. The levels of secreted precursor α-factor indicated are the means of two independent experiments; SD < 15%.