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The intracellular localization of a PtdIns(4)P-specific FLARE to the Golgi is dependent on Pik1p activity. Wild-type cells (SEY6210), pik1ts cells (AAY104), mss4ts cells (AAY202), or arf1Δ cells (6210arf1Δ) expressing GFP-PH(FAPPI) were incubated at 26°C (left column) or 38°C (right column) for 20 min as indicated and observed by fluorescent microscopy using a DeltaVision deconvolution system. DIC, Nomarski optics.
