Table 2.
Phosphoinositide levels in wild-type and various PI phosphatase and kinase mutant cells
| Strain | PtdIns levels (% of total 3H-labeled PI)
|
|||
|---|---|---|---|---|
| PtdIns(3)P | PtdIns(4)P | PtdIns(3,5)P2 | PtdIns(4,5)P2 | |
| sac1tsf sjl2Δ sjl3Δ 38°C | 0.75 ± 0.09 | 27.4 ± 3.9 | 0.80 ± 0.13 | 0.97 ± 0.17 |
| sac1tsf sjl2Δ sjl3Δ (CEN sjl2C446S)a 38°C | 0.88 ± 0.12 | 28.1 ± 4.4 | 0.75 ± 0.16 | 1.04 ± 0.11 |
| sac1tsf sjl2Δ sjl3Δ (CEN SJL2)a 38°C | 0.68 ± 0.15 | 12.0 ± 1.7 | 0.27 ± 0.09 | 1.18 ± 0.24 |
| sjl1Δ sjl2ts sjl3Δ 38°C | 0.90 ± 0.10 | 1.21 ± 0.12 | 0.20 ± 0.04 | 2.93 ± 0.30 |
| sjl1Δ sjl2ts sjl3Δ (CEN sjl2D850S)b 38°C | 0.87 ± 0.13 | 1.10 ± 0.11 | 0.10 ± 0.03 | 2.78 ± 0.16 |
| sjl1Δ sjl2ts sjl3Δ (CEN SJL2)b 38°C | 0.94 ± 0.11 | 0.80 ± 0.10 | 0.10 ± 0.02 | 1.30 ± 0.10 |
| Wild type 26°C | 0.88 ± 0.03 | 0.99 ± 0.08 | 0.05 ± 0.01 | 0.89 ± 0.05 |
| Wild type 38°C | 0.85 ± 0.04 | 1.14 ± 0.10 | 0.08 ± 0.02 | 1.28 ± 0.14 |
| mss4ts 26°C | 0.86 ± 0.08 | 0.88 ± 0.06 | 0.02 ± 0.01 | 0.51 ± 0.06 |
| mss4ts 38°C | 0.79 ± 0.05 | 1.05 ± 0.08 | 0.09 ± 0.01 | 0.17 ± 0.05 |
| sjl1Δ sjl2Δ 26°C | 0.69 ± 0.12 | 0.91 ± 0.11 | 0.04 ± 0.02 | 1.90 ± 0.26 |
| sjl1Δ sjl2Δ mss4ts 26°C | 0.74 ± 0.07 | 0.86 ± 0.09 | 0.06 ± 0.02 | 0.94 ± 0.13 |
Yeast strains incubated at the indicated temperatures were labeled with myo-[3H]inositol. Lipids were extracted and deacylated for analysis by HPLC as described in Materials and Methods. The mean peak area (cpm) of each PtdIns species is reported as a percentage of total 3H-labeled PIs. The values reported are the means (± SD) of at least two or three independent experiments. The appropriate PI species in each subset of experiments have been indicated in bold text to highlight relevant comparisons.
Derived from AAY143 (Foti et al., 2001) by transformation with pRS415SJL2 or pRS415sjl2C446S.
Derived from YCS176 by transformation with pRS415SJL2 or pRS415sjl2D850S.