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. 2002 Feb;13(2):579–592. doi: 10.1091/mbc.01-03-0140

Figure 11.

Figure 11

Expression of GFP fusion of domain III of EF1α or EBS of Bni1p inhibits the localization of β-actin mRNA to the protrusions in vivo. Transfected cells were identified by their green fluorescence. Cells with concentrated mRNA in protrusions were scored as localized, and those with perinuclearly or uniformly distributed mRNA were scored as nonlocalized. The percentage of cells with localized mRNA was calculated for each experiment and summarized in A. Lane 1, β-actin mRNA in untransfected (275 cells); lane 2, β-actin mRNA in cells expressing GFP (231 cells); lane 3, β-actin mRNA in cells expressing GFP-domain III (308 cells); lane 4, β-actin mRNA in cells expressing GFP-EBS of Bni1p (349 cells); lane 5, α-tubulin mRNA (a nonlocalized mRNA) in untransfected cells (557 cells); lane 6, β-actin mRNA in untransfected but serum-starved cells (2460 cells). Bars, SD; lanes 1–3 and 5, n = 3; lane 4, n = 2; lane 6, n = 10. (B) Representative cells expressing GFP-EBS of Bni1p or GFP-domain III of EF1α have a uniform distribution of β-actin mRNA, whereas most of untransfected cells localize β-actin mRNA to protrusions. (a and c) GFP fluorescence indicating transfected cells (marked with asterisks); (b and d) β-actin mRNA (arrowheads indicate β-actin mRNA localization to the protrusions). Bar, 20 μm.