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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Mol Cancer Ther. 2019 Apr 8;18(6):1045–1056. doi: 10.1158/1535-7163.MCT-18-0146

Figure 3: USP14 inhibitor b-AP15 suppresses cell proliferation, causes accumulation of ubiquitinated proteins and induces UPR in NB.

Figure 3:

A. b-AP15 showing cytotoxic effect on NB cells. Six NB cell lines IMR-32, NGP, SK-N-BE2, SH-SY5Y, SK-N-AS, and LAN-6 were incubated with serial dilutions of b-AP15 or medium alone for 72 hours. Cell Counting Kit-8 (CCK-8) assay was used to determine cell viability. B. IC50 values of six cell lines tested were measured based on CCK-8 assay. C. Morphological changes of six NB cell lines after incubation with serial dilutions of b-AP15 for 72 hours were shown. D. Two USP14-knockdown cells, NGP and SH-SY5Y, were harvested, and subjected to immunoblotting with anti-Lys48-linked specific antibody. Same amounts of lysates were performed Coomassie Blue staining as loading controls. E. NGP and SH-SY5Y cells were treated with b-AP15 (1 μM) for the indicated time points. Cell lysates were subjected to immunoblotting as the above. F, G. USP14 knockdown NGP and SH-SY5Y (F) cells or b-AP15 treated NB cells (G) were subjected to immunoblotting with anti-HSP40, HSP70, and USP14 antibodies.